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文献和实验/GFP is U54830 . A kit for mutagenesis of a yeast gene with mTn-3xHA/GFP is available. See what the kit contains Order the kit Protocols for shuttle mutagenesis/epitope-tagging of a yeast gene with mTn-3xHA
on ice for 30 min (all antibodies 1/100 v/v). 4. Wash with 10 mL of PBS/FBS, spin and resuspend in PBS/FBS with 1 µg/mL of propidium iodide. Filter through 70 µm nylon mesh. 5. Select cells which are PI negative, c-kit positive, Sca-1 positive
Genome‐Wide Annotation and Quantitation of Translation by Ribosome Profiling
100 U/µl RNase I 20 U/µl SUPERase‐In (Life Technologies) 1 M sucrose cushion (see recipe ), ice cold
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