VMRD特约代理商|S1D12 PEDV MAb Cell

Purification of Lin-, c-kit+, Sca-1+ bone marrow cells for Culture, Flow Cytometry, or Transplantaion

. Cell Sorting (Lin-,cKit+,Sca1+): 1. Incubate cells in FcgRII block on ice for 10 min. 2. Stain an aliquot and with PE-IgG2a and FITC-IgG2b isotype antibodies as neg. controls. Incubate remainder of cells with Sca-1 PE, plus c-kit FITC antibodies

Combined Flow Cytometric Measurement of Two Cell-Surface Antigens and DNA-RNA Content

) directed against cell surface antigens of interest (biotinylated or labeled with an appropriate fluorochrome), and isotype-matched controls The protocol described here uses one biotinylated mAb in combination with streptavidin-Alexa Fluor 488

Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional Profiling

Mouse IgG2a isotype control (0.5 mg/ml; BD Pharmingen, cat. no. 554121) Mouse IgM isotype control (0.5 mg/ml; BD Pharmingen, cat. no. 553472