Epicentre特约代理商|CL4111K CircLig

SMART™ cDNA 技术问答

有SMART cDNA 合成和文库构建到? TriplEx2 或者Creator 供体载体 pDNR-LIB所需的试剂。因为该试剂盒是特别针对文库构建来设计的，所以它与Clontech™ PCR-Select™cDNA 差减杂交试剂盒不能配合使用。 SMART PCR cDNA Synthesis Kit 可以用来与Clontech™ PCR-Select™ cDNA 差减技术一起使用。总RNA 不能直接用来做差减实验，但可以通过SMART PCR cDNA Synthesis Kit 来扩增出

Detection of Reverse Transcriptase Termination Sites Using cDNA Ligation and Massive Parallel Sequencing

on ligation of an adapter to the 3′ end of cDNA with bacteriophage TS2126 RNA ligase (CircLigase™). In the following PCR amplification, Illumina adapters and index sequences are introduced, thereby allowing amplicons to be pooled and sequenced on the standard

BioBrick Assembly Using the In-Fusion PCR Cloning Kit

™ assembly normally involves assembly of two BioBricks™ at a time using restriction enzymes and DNA ligase. Here we describe an alternative BioBrick™ assembly protocol that describes the assembly of two BioBricks™ using the In-Fusion PCR Cloning Kit