bethyl特约代理商|E10-128 Bovine alp

Detection of Antibodies to Self-Antigens (K-alpha 1 Tubulin, Collagen I, II, IV, and V, Myosin, and Vimentin) by Enzyme-Linked Immunosorbent Assay (EL

The enzyme-linked immunosorbent assay (ELISA) is a widely used technique for detecting antibodies (Abs) and is employed in clinical laboratories to identify Abs against various self-antigens–autoAb development and quantitation. This method

Sandwich ELISA Protocol

4 2.0 g KCl Add ddH2O up to 10 L, pH to 7.2 with HCl PBS/Tween: 0.5 ml of Tween-20 in 1 L PBS Blocking Solution: 10% fetal bovine serum or 1% BSA in PBS. Filter before use to remove particulates

ELISA操作指南

There are several other methods to wash an ELISA plate.Whatever you choose, be consistent with your washing technique throughout the whole process.        ELISA板还有其它几种洗涤方法。无论使用哪种方法，请在整个ELISA实验过程中保持一致 Phase 2: Blocking non-specific binding        第二阶段：封闭非特异性结合 Bovine