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Synthesis of (2S, 4S, 5S)-5-(t-Butoxycarbonylamino)-4-(t-Butyldimethylsilyloxy)-2-Isopropyl-7-Methyl Octanoic Acid

In an effort to produce renm mhibttory peptides, the preparation of (2 S ,4 S ,5 S )-5-( t -Butoxycarbonylamino)-4-( t -butyldimethylsilyloxy)-2-isopropyl-7-methyl octanotc acid, 15 , was carried out ( 1 ). This pseudo-dipeptide was used

Phosphoamino acid analysis:Mark Kamps's method

more than 3 µl is tedious,so don't use much H20.On the other hand,I wouldn't use much less than 6 µl so as to have good recovery.   7.Spot 1 µl PAA standards (about 0.3 µg each of PSer,PThr,and PTyr)on a cellulose thin layer chromatography plate and then spot

Phosphoamino acid analysis:Mark Kamps method

Phosphoamino acid analysis:Mark Kamps's method 1. Label your protein with 32Pi. Then subject the protein to SDS polyacrylamide gel electrophoresis and transfer your gel-fractionated protein to Immobilon-P .