DAKO特约代理商|S3022 S302281-2|Anti

聊一聊血清学研究中的表面封闭剂

backgrounds, like in.vent Diagnostica GmbH in Hennigsdorf/Berlin, Germany. Figure 1 illustrates how poorly a simple BSA surface blocker performs in terms of background signal variation in contrast to CANDOR’s The Blocking Solution in a simple comparison test

DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE

). In most cases, background activities can be minimized with heat and chemical inhibitors that leave PLAP active (Zoellner and Hunter, 1989). In addition, monoclonal and polyclonal antibodies specific to PLAP can be purchased (Dako, Zymed, Medix, and Accurate

Kamps's Western Blotting Protocol

antibodies,longer washes reduce the signal,shorter washes increase the background. 8)Incubate the filter for 30 min with 125I-protein A in 3% BSA in rinse buffer (You can re-use your blocking buffer for this).We use ICN's 68038 (approx.30mCi/mg specific