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文献和实验【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
overnight. Note. For multiple samples, prepare a master mix. 7. Purification: purify the ligation reaction with QIAquick PCRPurification Kit as described in step 2. 8. Primary PCR: In 0.2 mL thin-wall PCR tubes, compose thefollowing components
Gene Knockout with Conventional Mutagens
) 39.1 ul water ------- 48 ul Put 48 ul A in each of 96 thin-wall 200 ul tubes. Add 2 ul 1:5 diluted DNA to each tube. Run PCR program (MJR PTC-200): Control method: CALCULATED 1: 92 degrees forever, beep. 2: 92 degrees
Endothelial Cell Systems - Instructions For Use
or call your formulation Technical Specialist for assistance. EGM ® & EGM BulletKit® (Endothelial Growth Medium) Basal medium based on modified MCDB-131, developed for normal human endothelial cell in a low-serum environment. EGM® is supplemented growth medium
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