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- 文献和实验
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上海玉博生物科技有限公司
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文献和实验E.Z.N.A.TM Plant DNA Maxi Protocol For Dry Specimens
x g 2. Centrifuge with swinging-bucket rotor at room temperature capable of 4000xg. 3. Nuclease-free 50 ml high speed centrifuge tubes (such as Nelgen polycarbonate tube Cat#3118-0050) and 50ml centrifuge tubes capable of 4000 x g
free shotgun sequencing library from miniprep BAC DNA
Miniprep of BAC DNA 1.Inoculate 4 - 8 tubes of 3ml/tube LB with 15μg/ml chloramphenicol with BAC clone and grow oveernight. Miniprep with AutoGen 740 according to the protocol described in our Web page. DNA pellet were resuspended in 265 μl TE
freeshotgun sequencing library from miniprep BAC DNA
. This mixture was sonicated for 2 - 3 seconds at 50% power in a Branson sonifier 250 on ice. For end repair, add 4 μl of 10 units/μlMung Bean Nuclease ( New England BIoLabs, #250 ), incubate for 25 minutes at 30℃. Purify DNA by phenol extraction/EtOH
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