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文献和实验8 The Recovery of Immunoglobulin Sequences from Single Human B Cells by Clonal Expansion
chain reaction (PCR) amplification of their variable-region genes, thereby immortalizing the VH and VL regions from individual human B cells. The method relies on the clonal expansion of single B cells in which cell-cell interactions (CD40-CD
Isolation, Enumeration, and Expansion of Human Mesenchymal Stem Cells in Culture
Human bone marrow (BM) contains a population of non-hematopoietic stem cells also termed stromal cells, mesenchymal cells or multipotent mesenchymal stromal cells (MSCs). These cells have unique stem cell-like properties including
Primary Culture of Human Adipocyte Precursor Cells: Expansion and Differentiation
cell lines are valuable tools for the identification of mechanisms associated with proliferation or differentiation such models do not necessarily represent the complexity of adipose tissue physiology. Primary cell culture systems may be closer
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