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文献和实验Production and Characterization of Recombinant Human and Murine TNF
and the identity of the final products can be checked by SDS-PAGE, ELISA, Western blot, analytical gel-filtration chromatography, mass spectrometry, and lipopolysaccharide assay. The biological activity of both human and murine TNF is assessed by in vitro cytolytic
Assay (Invitrogen) as suggested by Illumina. Illumina Bead Array MicroRNA Detection Starting with 500 ng/sample of total RNA, mature microRNAs were amplified with the Illumina human v1 MicroRNA expression profiling kit containing primers for 743
) abs510003-96T Human IL-6 ELISA Kit abs510006-96T Human TNF-α ELISA Kit abs510002-96T Human IL-1β ELISA Kit abs510004-96T Human IL-8/CXCL8 ELISA Kit abs520003-96T Mouse IL-4 ELISA Kit abs520006-96T Mouse IL-12 p70 ELISA Kit abs520007-96
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