ebioscience特约代理商|BMS223-4 TNF

Production and Characterization of Recombinant Human and Murine TNF

and the identity of the final products can be checked by SDS-PAGE, ELISA, Western blot, analytical gel-filtration chromatography, mass spectrometry, and lipopolysaccharide assay. The biological activity of both human and murine TNF is assessed by in vitro cytolytic

MicroRNA Expression Profiling by Bead Array Technology in Human Tumor Cell Lines Treated with Interferon-Alpha-2a

Assay (Invitrogen) as suggested by Illumina. Illumina Bead Array MicroRNA Detection Starting with 500 ng/sample of total RNA, mature microRNAs were amplified with the Illumina human v1 MicroRNA expression profiling kit containing primers for 743

肿瘤坏死因子-α(TNF-α)生物学活性测定

　　肿瘤坏死因子-α(TNF-α)是一种主要由单核-吞噬细胞产生的单核因子，不仅具有选择性地杀伤某些肿瘤细胞，而且有多种免疫调节作用。其检测方法主要包括生物学活性测定和免疫学检测方法。其中细胞毒生物学检测方法敏感性较高，最为常用；免疫学检测方法包括酶联免疫吸附试验(ELISA)和放射免疫测定法。 　　(一)　原理: 　　TNF-α受体广泛地分布于多种肿瘤细胞和血