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上海玉博生物科技有限公司
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
dilutions for rabbit polyclonal antibodies range between 1:50 and 1:500. For double-labeling experiments, use primary antibodies raised in two different species. Antibodies, secondary Secondary antibodies are diluted in PBS containing 2% normal
1. 取蜡块切片15-20张(8UM),置于EP管中,加入1ML的二甲苯,37度作用3小时,10000×3分钟,倾去二甲苯。重复3-4次,观察管壁是否光滑。若有蜡质残存,需继续脱蜡。 2. 剃度酒精水化:100% 1ML,室温30分钟,10000×3分钟,去上清;95%;75%;50%。 3. 消化:1×SSC 500UL ,洗涤沉淀,12000×3M离心。干燥沉淀后加石蜡消化液 400UL,20mg/ml的PK 20ul;55度消化120小时,至第四天,补加PK 10
PMSF (1:1000 vol/vol of PMSF stock solution)) for about 5 ml per rat brain. Critical step Stir the buffer thoroughly while slowly adding PMSF stock solution, as PMSF might precipitate otherwise. Kill 10�40 rats and remove their brains
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