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文献和实验plates #TF-0384) or 16.3ml per plate, if PCR volume is 40µl/well (maximum for new ABT-384 plates #AB-0937). Add the enzyme last. We were told that Pfu polymerase can destroy primers if PCR mix stored for too long at room temperature
plates #TF-0384) or 16.3ml per plate, if PCR volume is 40µl/well (maximum for new ABT-384 plates #AB-0937). Add the enzyme last. We were told that Pfu polymerase can destroy primers if PCR mix stored for too long at room temperature
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
, set the pulse frequency to 100, continuous mode, and reduce power to a minimum using the half-wave plate. Use a Post-It note or lens paper to visualize the laser beam. 5) Adjust and fix the kinematically mounted mirrors in sequence
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