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上海玉博生物科技有限公司
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文献和实验Anti-BrdU-FITC (Becton Dickinson or Phoenix Flow) 0.15 M NaCl, 1.5 M NaCl 10 Paraformaldehype (kept as stock in -80°C) Tween 1M MgCl2 FACS Tubes Protocol: Cells in 96 well FACS plate Block with 24G-2 Surface stain cells
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
) Dissect embryos or small pieces of tissue (e.g. the size of a retina from an adult rat works well with this procedure) into PBS containing 2mM MgCl2 (PBS + Mg2+) on ice. PBS(10X) 80 g NaCl 2 g KCl 11.5g Na2HPO4 2 g KH2PO
membrane proteins (see protocol 8 and ) Native standard proteins (e.g., in the range of 100‐ to 1000‐kDa) Sample buffer (see reciperecipes ) Anode
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