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上海玉博生物科技有限公司
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文献和实验using the AllPrep DNA/RNA 96 Kit. Real-time RT-PCR analysis of the PGK1 transcript was performed using a primer-probe set that could amplify and detect both mRNA and genomic DNA sequences. The flat amplification plot for the no RT control (-RT) indicates
SequalPrep Long PCR Kit with dNTPs
Volume Final Concentration SequalPrep™ 10X Reaction Buffer 2 μl 1X DMSO
for 60 minutes. 5) Add 100 μl FP3 Buffer and vortex to mix. 6) Store the extraction at 2-8°C. 2. PCR Protocol This protocol serves as a guideline for PCR amplification. Optimal reaction conditions, such as incubation times
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