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文献和实验Negative Staining for Transmission Electron Microscopy
and must be manipulated with care. They are more stable if supported on specimen grids with small mesh size (e.g. 400 mesh). Hexagonal grids seem to offer a more stable support. Making the carbon film Take a piece of mica and cut
. The stain is rinsed off with distilled water and the sections are dried and covered with a glass coverslip using a synthetic mounting medium such as D.P.X. THIN SECTIONING FOR ELECTRON MICROSCOPY The sections are cut
Negative Stain Electron Microscopy of Microtubules
and opening up of microtubules into flat sheets are common. Cryo-electron microscopy, where microtubules are flash frozen in a thin film of vitreous ice and imaged without staining, is currently regarded as the best method to view native microtubule structure
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