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上海玉博生物科技有限公司
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文献和实验Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.
in liquid nitrogen or at –80°C for later use. ii. Add the appropriate volume of Lysis/Binding Buffer to either a frozen cell pellet or to a fresh cell pellet. Perform a repeated passage of the solution through a pipette tip
application. 3. RNA Storage Store the purified RNA on ice for immediate use. For long–term storage, keep the purified RNA at –80°C. Perform DNase I treatment after purification (refer to the PureLink™ RNA Mini Kit manual) to assure highly
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