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文献和实验Gene Knockout Techniques(1) The Knockout Process
with seeded agarose). We do everything in multiples of 96 if possible; it is usually sufficient to set up between 192 and 384 wells. When the wells starve, harvest in the same way that library plates are harvested, cook the worms, inactivate the Proteinase K
machines with 96- or 384-well format blocks. Somehave interchangeable blocks. Reactions can occur in singletube, strips, 96- or 384-well plates, or capillaries. Reactionvessel volumes for use in conventional PCR are typically 0.2 or0.5 mL. Ramp rates
RASL‐seq for Massively Parallel and Quantitative Analysis of Gene Expression
, quantitative analysis of gene expression can be accomplished by microarray or RNA?seq analysis. While these methods are applicable to genome?wide analysis, it is often desirable to quantify expression of a more limited set of genes in hundreds, thousands
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