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ADP-Ribosylation of -Gi Proteins by Pertussis Toxin: Positional Dissection of Acceptor Sites Using Membrane Anchored Synthetic Peptides

Bordetella pertussis , the causative agent of whooping cough, secretes a plethora of virulence factors contributing to the onset and characteristic of the disease. The exotoxin pertussis toxin (PT) is regarded as particularly important

Genetic Detoxification of Bacterial Toxins

Several pathogens, such as Corynebacterium diphtheriae, Clostridium tetani, Bordetella pertussis, Vibrio cholerae , enterotoxigenic Escherichia co1i (1 ), and even some emerging pathogens, such as Helicobacter pylori (2 ), produce potent

Pyrosequencing在分子诊断方面的应用

到室温。退火后，为了在Pyrogram™中更加容易分辨结果，再加入2.2ug单链DNA聚合蛋白（SSB, Amersham Biosciences）。 使用Pyrosequencing™技术进行序列分析：     样品使用带有SQA软件的PSQ 96系统和SQA试剂盒（Pyrosequencing AB）进行序列分析。 结果：     设计序列分析以涵盖toxin S1的一个短的区域，并区分B.pertussis的四个不同的等位