MIDSCI特约代理商|TP87050 TPP 50 mL
Bioreactor Increase cell productivity and growth by allowing for efficient gas exchange and even dispersal of nutrients- ￥3000
- merck
- 5.30509.0001
- 2025年07月06日
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上海玉博生物科技有限公司
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文献和实验b-Galactosidase Activity Assay
to mid-log.1 Prepare solutions: Z buffer, phosphate buffer, ONPG2 .Preparation of Cells Incubate cultures 20' on ice to stop growth and wash:Pellet at least 2 mL of cells at 4 C by centrifuging 10' at 6,000 rpm in a Sorval SS34 rotor.Pour off the supernatant
Dynamic Monitoring of Cellular Remodeling Induced by the Transforming Growth Factor-β1
. Fig. 4 Inhibition of formation of contractile microfilaments by cytochalasin B (CB) leads to a rapid drop of cell index values. BPH-1 cells were pretreated with transforming growth factor-β1 (10 ng/ml) for 68 h and treated with CB (10 μg/ml
. No. D8537) using a volume equivalent to half the volume of culture medium. Repeat this wash step if the cells are known to adhere strongly. Pipette trypsin/EDTA (Prod. No. T4049) onto the washed cell monolayer using 1ml per 25cm2 of surface area
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