Qiagen特约代理商|981005 PCR Tubes,

Preparation of total RNA from whole blood collected in PAXgene tubes

top centrifuge.2.Remove the supernatant by decanting or pipetting. Add 5 ml RNase-free water to the pellet, and close the tube using a fresh secondary Hemogard closure.Gently decant the supernatant, and blot on a paper towel.3.Thoroughly resuspend the pellet

PCR 不必“摸条件”

μL 体系 2× MasterMix 25 μL 上游 Primer （ 10 μM 1-5 μL 下游 Primer （ 10 μM ） 1-5 μL 模板 lamid DNA ～ 50 ng genomic DNA ～ 1000 ng cDNA ～ 500 ng 粗提液 ～ 2 μL 灭菌蒸馏水至 50 μL PCR 扩增实例： 一、plasmid DNA 扩增 1．提取的质粒 DNA 扩增 以pUC19 质粒为模板扩增 100，250，500

Troubleshooting for PCR and multiplex PCR

the amount of PCR primer　　Increase the amount of DNA template　　Increase the amount of Taq polymerase　　Change buffer (KCl) concentration (higher if product is lower than 1000bp or lower if product is higher than 1000bp)　　Add adjuvants. Best, use BSA (0.1 to 0.8 µg/µL