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文献和实验CNV Analysis Using TaqMan Copy Number Assays
reporter dye attached to the 5′ end, a nonfluorescent quencher (NFQ), and a minor groove binder (MGB) attached to the 3′ end (MGBs increase the melting temperature (T m ) without increasing probe length and allow the design of shorter probes
membrane with UV crosslinker. Blot off extra liquid, then roll membrane and place in hybridization tube with DNA side inward. Prehybridize with 15 ml Rapid Hyb™ 15-30 minutes at 65°C. Hybridize Note: Make probe either that morning
Use of Channelrhodopsin for Activation of CNS Neurons
is threaded onto the optical fiber to protect the connection. View Image Figure 2.16.2 Steps
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