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文献和实验Studying Wound Repair in the Mouse
of wound healing provide vital insights into the mechanisms and pathophysiology of cutaneous wound repair and are a crucial part of clinical research into the development of new strategies and approaches to rational wound therapy. Although considerable
ul add 1 ul of RNasin and speedivac down to 18 ul without heat. Heat to 70o C for 10 min. Cool on ice for 1 min. Prepare the following: 8 ul 5x RT buffer
freeshotgun sequencing library from miniprep BAC DNA
of DNA from agarose gel ( Bio 101, #3105). 9.Sonication and end-repair DNA pellet were resuspended in 100 μl ddH2 O and added 20 μl 10X MB buffer( 300 mM NaOAc pH5.0, 500 mM NaCl, 10mM ZnCl2 , 50% Glycerol), mixed in an Eppendorf tube
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