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文献和实验Immunohistochemistry using Anti-Ganglioside Antibodies
supernatant containing antiganglioside mAb overnight at 4°C. The time of incubation may range from 30 min. to overnight as determined by experience. 5. Wash with 1% BSA in PBS 4 times. 6. Incubate in FITC-conjugated goat anti-mouse IgM F(ab
Dual- and Triple-Color Fluorospot
for the other cytokine (Fig. 1). As a second step in the detection, streptavidin conjugated to a red fluorophore (Cy3) and anti-FITC antibodies labeled with a green fluorophore, respectively, are used (10, 11). The resulting spots are subsequently analyzed using
Flow Cytometric Analysis Of Bcl Family members
against Bcl-xL (Santacruz Biotechnology, USA) and rabbit polyclonal against Bax (Santacruz Biotechnology, USA). The secondary AB against Bcl2 was goat anti mouse Ig FITC (Immunotech, USA) and against Bcl-xL and Bax goat anti rabbit FITC (ICN-Cappel, USA). FITC
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