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文献和实验Stable Transfection (Electroporation)
medium. Supplies & Equipment: BioRad gene pulser with capacitance extender Electroporation cuvettes with 0.4 cm interelectrode distance Reagents: sterile 1 x HeBS buffer (Hepes buffered saline) G 418 (Geneticin
Transformation of E. coli by Electroporation
for electroporation. 1. Use a fresh colony of DH5α (or other appropriate host strain) to inoculate 5 ml of SOB (without magnesium) medium in a 50 ml sterile conical tube. Grow cells with vigorous aeration overnight at 37EC. 2. Dilute 2.5 ml of cells into 250
Mesoplasma florum:Electroporation
level. Select cultures which are just changing color. chilled mycoplasma electroporation buffer 8 mM HEPES pH 7.4 272 mM sucrose chilled 1 mm electroporation cuvettes 10 ml chilled ATCC 1161 medium
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