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文献和实验, change 2 x daily). 10) Centrifuge dialysed collagen at 30,000 x g for 1.5 hrs. 11) Remove supernatant and place in sterile flask. 12) Adjust the collagen I concentration to ˜2 mg/ml using 0.5 mM acetic acid at 4°C.
V294.PART II,Chapter 2 Boyden法体外趋化试验
9791; Sigma-Aldrich): dissolve collagen at 1mg/mL in 0.1 N acetic acid. Allow to stir at room temperature until dissolved(takes 1–3 h). Keep collagen stock at 4°C. 9. Methanol. 10. Giemsa stain, modified solution (cat. no. GS500; Sigma-Aldrich
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
reaction buffer can be stored for at least one year at room temperature in foil-covered container. X-gal stock(40X) 40 mg/ml X-gal in dimethylformamide Store at -200 C in foil covered glass container. Rinse many times in PBS
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