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文献和实验Bromodeoxyuridine Staining of Mammary Tissue
and incubate sections overnight at 4oC with anti-BUdR-POD antibody (Boehringer Mannheim Cat.# 1 585 860) diluted 1:15 in PGB diluent. 11. Rinse sections 3 x 5 min in PBS. 12. Incubate with unactivated DAB for 5 minutes followed by activated DAB for 10 minutes
免疫组化技术-Immunohistochemical Protocols
2) or a biotin conjugated (Table2) secondary antibody is used, both at a dilution of 1:500 in BSA diluent for 1 hr, Wash in PBS 3x5 min Incubate in avidin-biotin-peroxidase complex (ABC reagent
100μl of standard or sample to each well in triplicate. Incubate at room temperature for at least 2 hours. 2) Detection: Wash plate four times. Dilute detection antibody (biotinylated) in diluent to a concentration of 0.5μg/ml (500ng/ml
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