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Purification of Lin-, c-kit+, Sca-1+ bone marrow cells for Culture, Flow Cytometry, or Transplantaion

A  c-Kit (2B8) FITC-IgG2b, 01904D  FITC-IgG2b Isotype control, 11184C  Fc RII block (clone 2.4G2), 01241D   Media: Heat inactivated FBS (56°C x 30 min) PBS + 2% heat-inactivated

Immunofluorescent Staining of Mouse and Rat Leukocytes

cells from murine peripheral blood or a spleen cell suspension can be lysed using BD Biosciences Pharmingen's PharM Lyse™ (Cat. No. 35221E) solution. Add 2.0 ml of 1X Lysing Solution to the spleen cell suspension or per 200 µl of murine peripheral blood

Purification of mAb (IgG)

(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10