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文献和实验The final working concentration of ROX Standard in the PCR buffer is 0.5 µMolar (1X). SYNTHEGEN's ROX Standards are shipped in liquid form at a concentration of 100 µMolar (200X).
Standard hybridization technique
The standard solution typically used for both pre-hybridisation and hybridisation is based
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
. autoclave, cool, and add: 1x MS vitamines (SIGMA #M-7150). Take 1ml of 1000x stock prepared by disolving 10.3gr in 100ml of water. antibiotic (kanamycin 50mg/l). Top agar: 1x Murashige&Skoog salts. 1% sucrose. adjust pH
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