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文献和实验Two-hybrid analysis of genetic
).The first application of two-hybrid methods to probe protein function was to examine the interactions between proteins isolated by two hybrid methods and relatively small numbers of test proteins (see for example,Durfee et al.,1993; Gyuris et al.,1993; Harper et al
Treatment of Liver Tumors in Rabbit
comprised between the electrodes. In the case of rather small and prominent cutaneous or subcutaneous tumors, it is easy to place two external electrodes on each side of the tumor nodule to be treated and to transcutaneously deliver the permeabilizing
CONVENTIONAL TWO STEP FIXATION [See D.H. Hall, 1995]
7. 3 rinses buffer (0.1 NaAcetate) 5 min. each 8. 3 rinses in distilled water, leave overnight, embed cut pieces in 3% seaplafue agarose 9. Cut out small agarose blocks containing worms; should be large enough to be excluded from pipette tip, but small
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