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Cell  Isolation  Techniques

should be taken from the refrigerator at least a half hour before opening, and they should be left in a dessicator. Before opening any of the vials, be sure it is not at all cool to the touch. All of the cell isolation enzymes cited in this section

ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES

medium, then plate all onto a 10cm dia petri dish containing NeoPMEFs and 10ml medium. Label plate 2. 5. Set up following control plates. a) Take 0.167 ml (2 x 106 cells) original cell suspension _ 10cm dia petri dish containing NeoPMEF's and 10

Methods for DNA isolation

DNA isolation The standard method for the miniprep isolation of plasmid DNA includes the same general strategy as the large scale isolation. However, smaller aliquots of antibiotic containing liquid media inoculated with plasmid-containing cell