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Mag-Bind Blood DNA Maximum Yield Protocol (2ml-6ml)

实验试剂   1. Absolute ethanol (96%-100%) 实验设备   1. Nuclease-free 50 ml centrifuge tube 2. Water bath, incubator or heating block preset at 65°C 3. Magnetic separation device for 50 ml tube

Mag-Bind Blood DNA Maximum Yield Protocol (7ml-10ml)

实验试剂   1. Absolute ethanol (96%-100%) 实验设备   1. Nuclease-free 50 ml centrifuge tube 2. Water bath, incubator or heating block preset at 65°C 3. Magnetic separation device for 50 ml tubes

Purification of Plasmid from 50 ml-culture

1. Shake E. coli harboring plasmid at 37 C overnight in 50 ml of TB containing appropriate antibiotics. (when using ampicillin, addition of the antibiotics to 100-200 ug/ml rather than usual 50 ug/ml may improve the yield of plasmids.)