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上海玉博生物科技有限公司
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文献和实验Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
Inc.) or QiaFilter™ (Qiagen Inc.) plate positioned over a 96 deep-well collection block inside a vacuum manifold. Transfer 600 ul at a time, collecting the top half (with flocculent ppt.) first. Vacuum filter using approximately 300-500 mm Hg until filtration
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
pipette tip, transfer the lysate to a Qiagen TurboFilter™ (Qiagen Inc.) or QiaFilter™ (Qiagen Inc.) plate positioned over a 96 deep-well collection block inside a vacuum manifold. Transfer 600 ul at a time, collecting the top half (with flocculent ppt
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmids*
before transferring to filter plate. 7. Using a wide bore pipette tip, transfer the lysate to a Qiagen TurboFilter™ (Qiagen Inc.) or QiaFilter™ (Qiagen Inc.) plate positioned over a 96 deep-well collection block inside a vacuum manifold. Transfer 600 ul at a time
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