chromsystems特约代理商|28039/XL 6PL

Essentials of Real Time PCR

has developed two types of chemistries used to detect PCR products using Sequence Detection Systems (SDS) instruments: • TaqMan® chemistry (also known as “fluorogenic 5´ nuclease chemistry”) • SYBR® Green I dye chemistry TaqMan® Chemistry The TaqMan

Real-Time RT-PCR

acquiring the instrumentation required for real-time analysis, this technique is becoming the dominant RT-PCR-based quantitation technique. Real-Time PCR Chemistries Currently four different chemistries, TaqMan® (Applied Biosystems, Foster City, CA, USA