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文献和实验and of the data obtained are shown. Materials and Methods Rna Extraction RNA extractions were carried out with the RNeasy mini kit (Quiagen, Hilden, Germany), according to the manufacturer"s instructions. We normally used 2 x 105 Cell s
Semiquantitative RT-PCR analysis to assess the expression levels of multiple…
Extraction RNA extractions were carried out with the RNeasy mini kit (Quiagen, Hilden, Germany), according to the manufacturer"s instructions. We normally used 2 x 10 5 cells, but cell numbers ranging from 1 x 10 4 to 2 x10 6
【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
Bcl I at 48°C(see Notes 11–13). 2. Purification: Purify the digestion products (from step 1) withQIAquick PCR Purification Kit. According to the directionsprovided by the kit, add 250 mL Buffer PB to each sampleand apply the mixture to the mini
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