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文献和实验Isolation of micro-RNA (miRNA)
Collect sample Flash-freeze sample in liquid nitrogen, weigh sample Use 100 μl of solution D for every 10 mg or 1 × 106 cells. Homogenize sample in solution D The volume of solution D = Volume D Add 1/10
Dynabeads® mRNA DIRECT Micro Kit - mRNA isolation for RT-PCR amplification
and resuspend the beads in Lysis/binding buffer to the original volume. Aliquot 20 μl suspension to each sample tube. 2. Preparation of lysate from cultured cells and cell suspensions This protocol is recommended for samples containing up to 1 x
microscope. This will allow observation of the cells without opening or disturbing the growth. 16. Make cell density determinations at 10 X magnification using a square ocular grid, as explained in Chapter One for the determination of area. 17. Plot
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