Merck Millipore 代理商|MAB201B An

Dual- and Triple-Color Fluorospot

labeled with green fluorophore emitting light at the same wavelength as FITC, streptavidin (SA) labeled with Cy3, and if three cytokines are analyzed, a mouse anti-tag mAb labeled with Cy5 (Mabtech). 5. Mouse mAb anti-human CD28 at 0.1 mg/ml

Peptide Inhibition ELISA Protocol

Concentrated Sulfuric Acid88.0 ml      di-H2O B. Procedure Titer MAb supes at serial 5-fold dilutions to determine the concentration at which the titer begins to drop.  Dilute the MAb to one-half the above dilution in PBT

Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas

to the kappa 1a of Y3 (eg. DA which is kappa 1b) to allow later screening of myeloma light chain loss variants (if required). b) The final boost should be by the intravenous route, four days before the fusion, to ensure maximal activity in the spleen.