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文献和实验growth medium to each tissue culture dish. Incubate at 37oC in 5% CO2 for 2 – 24 hrs to allow the cells to adhere to the cover slips. Bring attached test cell lines into suspension using a cell scraper. Suspension cell lines may be tested directly
characteristics, depending on the media, the temperature and the strain of cells utilized. For bacteria (and some algae, fungi and other eukaryotic tissue culture) it is possible to measure the growth of cell populations by calculating cell number or mass
TYPES OF CELLS GROWN IN CULTURE Tissue culture is often a generic term that refers to both organ culture and cell culture and the terms are often used interchangeably. Cell cultures are derived from either primary tissue explants or cell
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