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文献和实验Replication timing by density transfer
, leu2-3,112, ura3-52, his6 in an A364A background. I aim for about 32 x 106 cells per time point. That gives enough DNA for at least 3 blots, leaving enough leeway for errors. 2. When the cell density is 2 x 106 cells/ml (OD660 ~ 0.16
74.1 23.06% 5.0 280.6 1.217 200 10% 1.94 109 1.09 515.5 Ammonium hydroxide NH4 OH 35.0 28% 14.8 251 0.898 67.6 Acetic acid, glacial CH3 COOH 60.05 99.5% 17.4 1045 1.05 57.5 Acetic acid 36% 6.27 376 1.045 159
Expanding Mouse Ventricular Cardiomyocytes Through GSK‐3 Inhibition
) Ki67 (rabbit monoclonal, Abcam, cat. no. 16667, 1 mg/ml, 1:300) α‐Sarcomeric actinin (α‐SA, mouse monoclonal, Sigma, cat. no. A7811, 1 mg/ml, 1:250
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