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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of interest as the solvents can partially dissolve indigo. For glutaraldehyde-fixed mouse retina, which is approximately 250 microns thick, the following procedure was used. Dehydrate through graded ethanols (50%, 70%, 95%, 100%, 100%) for 20 min each. Clear in xylene, 2 x 15
【交流】染色体免疫沉淀技术详细步骤-young lab ！！！
to quench formaldehyde. 4. Rinse cells twice with 5 ml 1x PBS. Harvest cells using silicon scraper. 5. Pool cells in 50 ml conical tubes and spin at 1,350 x g for 5 minutes at 4°C (Sorvall Legend RT centrifuge with swinging bucket rotor). Discard
Single and Double FISH protocols for Drosophila
. 2. PBT solution: 1XPBS, 0.1% Tween-20. 3. PBTB solution: 1XPBS, 0.1% Tween-20 and 0.5% milk powder. 4. Mouse monoclonal anti-fluorescein antibody (IgG: 1/2000 dilution of a 0.1 mg/ml stock solution [see notes 2,3]; Boehringer Mannheim, Laval
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