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文献和实验Labeling Tubulin and Quantifying Labeling Stoichiometry
5 (and-6) carboxyfluorescein succinimidyl ester ( Molecular Probes C-1311) Oregon Green 488 carboxylic acid, succinimidyl ester 5-isomer ( Molecular Probes O-6147) 5 (and-6) carboxytetramethylrhodamine succinimidyl ester ( Molecular Probes C-1171
Preparation of probes for in situ hybridization
compound could be used. In practice, CY3 (1 vial of monoreactive labelling kit per reaction, Amersham), works very well for as a red fluorophore, and the succinimidyl ester of Oregon green 488 (1mg of compound per reaction, Molecular Probes) as a green dye
Preparation of probes for in situ hybridization
kit per reaction, Amersham), works very well for as a red fluorophore, and the succinimidyl ester of Oregon green 488 (1mg of compound per reaction, Molecular Probes) as a green dye. Labeling is then conducted for 24-48 h in the dark, at room
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