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文献和实验Attachment of Nitroxide Spin Labels to Nucleic Acids for EPR
.A., Richter, C., and Schwalbe, H. 2010. High‐resolution NMR structure of an RNA model system: The 14‐mer cUUCGg tetraloop hairpin RNA. Nucleic Acids Res. 38:683‐694. Obeid, S
Rhodamine-Phalloidin/Calcofluor Staining
4 + 2.4 grams KH2PO4. pH upon dilution to 1X should be 7.2. Rhodamine Phalloidin Molecular Probes Inc., Dissolved in methanol according to manufacturers instructions to ~6.6uM and stored dessicated at -20¡C. Cat#R-415. Calcofluor aka
(casamino acids, BSA, serum), and decreasing the amount of protein applied to the electrophoresis gel.• Occurrences of extra bands in the blot can be resolved by several strategies. Run a control blot omitting the primary antibody to determine
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