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文献和实验SQ Blood DNA Maxi Protocol for 4-10 ml whole blood
实验试剂 1. Isopropanol (100%) 2. 70% ethanol 3. Using different volume of Solution according blood volume as following: 实验设备 1. Centrifuge capable of 2,000 x g 2. Nuclease-free
BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
. Then, the supernatant of each tube is transfered into 50 ml Corning centrifuge tube and DNase-free RNase A is added to a final concentration of 100 ug/ml from a 20 mg/ml stock and RNase T1 is added to a final concentration of 40 ul/100 ml from a 100 U/ul stock followed
RNA Purification Protocol for 100-200 mg of Animal Tissue
设备 1. Centrifuge capable of 3,000-5,000 x g 2. High speed centrifuge tube with capacity of at least 15 ml 3. Ice bath 4. Ground pestle 实验步骤 1. Dissect tissue quickly and freeze in liquid nitrogen
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