- 询价
- 子科生物
- 深圳
- ZK-L2025
- 2025年08月21日
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—20℃,12个月
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50
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深圳子科生物
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文献和实验RNAse A Treatment of Mouse Cells
works perfectly for molecular biology applications but it can not be used for the cells, it simply kills them. comment 2 ). The protocol below allows to obtain RNAse A that is free of Dnase. Dissolve RNAse A at a concentration of 10 mg/ml
RNase Protection Assay (Beverly Faulkner-Jones)
. Store in aliquots at -20oC RNaseA 2 mg / ml of Sigma R5250 X-A in 10 mM Tris pH 7.5, 15 mM NaCl. Store small aliquots at -20oC RNase T1 100 mg / ml Sigma R1003 in 50% glycerol and 10 mM NaPO4 pH 6.5. Store small aliquots at -20oC. 10 x RNase digestion
Ribonuclease A (RNase A), DNase-free
guanidinium thiocyanate plus 0.1 M 2mercaptoethanol and heavy metal ions.Inactivated by phenol/chloroform extraction.Note The working concentration for RNase A is 1-100 µg/ml depending on the application. The enzyme is active under a wide range of reaction
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