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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Immunoflourescence of Cultured Cells
as in 4). 7) Add secondary antibody as in 5) and 6) above. (e.g. FITC-conj goat anti-rabbit (1:1000) or Biotinylated isotype specific anti mouse for immunoperoxidase staining). Wash as before. 8) To minimize quenching of flourochrome mount
Studies of the Ubiquitin Proteasome System
from other investigators) Transfection efficiency control plasmid, e.g., GFP, RFP, β‐galactosidase 2× HEPES‐buffered saline (HeBS; see recipe ) Anti– tag epitope antibody or anti‐substrate
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