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文献和实验Immunofluorescent Staining of Mouse and Rat Leukocytes
cold wash buffer (PBS/0.1% NaN3/1.0% fetal bovine serum). Centrifuge at 350 x g for 5 min. Finally, resuspend cell pellet to a concentration of 2 x 107 cells/ml (i.e., 106 cells per 50 µl). Dilute primary mAbs (e.g., unconjugated, biotinylated
) of 0.5 ml min-1, whereas unconjugated siRNAs had a t1/2 of 6 min and CL of 17.6 ml min-1. As measured by an RNase protection assay (RPA), chol-siRNAs showed broad tissue biodistribution 24 h after injection in mice. Although no detectable amounts
Antigen Design Sera Purification Tech Sheet
generation has secondary structure similar to the epitope and/or if the protein epitope has enough continuous sequence for the antibody to bind with a lower affinity. When examining a protein sequence for potential antigenic epitopes, it is important
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