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文献和实验Isolate It All：siRNA • miRNA • Total RNA • Native Protein
Figure 2. Analysis of Total RNA Isolated with the mir Vana ™ PARIS ™ Kit. Total RNA was isolated from 1 x 106 HeLa cells with the indicated kit as per protocol. (A) 1 µg of total RNA was resolved on a 15% denaturing acrylamide gel and stained
Inducing RNAi with siRNA Cocktails Generated by RNase III
a number of long dsRNA substrates. Human GAPDH, La, and c-FOS dsRNA (200 bp) was prepared by in vitro transcription ( Silencer ™ siRNA Cocktail Kit (RNase III); See Materials and Methods). The dsRNA was digested using 1 U RNase III
in your own lab to detect RNases and a protocol is provided in Technical Bulletin 166 to facilitate this. However this assay is time consuming and requires working with radioactivity. As an alternative, Ambion's RNaseAlert™ Kit (Cat #1964) allows researchers
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