stemcell特约代理商|07905 07905 Dulb

类器官培养与应用——卵巢癌类器官

基 解离培养基 OCO培养基 细胞冻存液 近岸蛋白产品货号 基础培养基 DMEM/F12 DMEM/F12 DMEM/F12 DMEM/F12   penicillin-streptomycin 2% - 1% -   FBS 10% - - 30%   Collagenase IV - 1mg/ml - -   L-glutamine - - 1×

whole mount preparations

Carnoy's Fix: 6 parts 100 % EtOH 3 parts CHCl3 1 part glacial acetic acid Carmine Alum Stain: Place 1 g carmine (Sigma C1022) and 2.5 g aluminum potassium sulfate (Sigma A7167) in 500 ml distilled water and boil for 20 min. Adjust final

v468 Chapter 2 Wnt活性蛋白分离及应用

penicillin (base)/mL and 10,000 μg streptomycin(base)/mL in 0.85% NaCl (liquid) (see Note 2 ). 3. Dulbecco’s phosphate-buffered saline (PBS). 4. Trypsin (0.25% liquid trypsin). 5. Cell culture dishes: 10- or 15-cm dishes or large surface areaculture