pierce特约代理商|17890 Tris/EDTA 1

Chromatin IP (CHIP assay)

7.5; 62.5 ml 4 M NaCl; 50 ml 10 % Triton X100; 5 ml 10 % NaDeoxycholate; protease inhibitors]  CHIP wash buffer: 10 mM Tris pH 8.0; 250 mM LiCl; 0.5 % NP-40; 0.5 % NaDeoxycholate; 1 mM EDTA. [ for 500 ml: 5 ml 1 M Tris pH 8.0; 5.3 g LiCl; 25 ml 10

常用缓冲液配制

at 4 C. Periodically dump the 1X TE and make up the fresh 1X TE from 10X stock. 10X TE (10:1) 1X 1L 1X 1L 10X 10mM Tris-HCl 1.06 g Tris-HCl 10.6 g 0.39 g Tris-Base 3.94 g 1mM Na2EDTA 0.475 g 4.75 g pH 8 at 25 C. Mix and store at 4 C

原创：western protocol

1.5M Tris(PH 8.8) 100ml 18.171g (at4°C) 10% SDS 100 ml 10g (at room temperature) 10% ammonium persulfate 1ml 0.1g(replace every week store at at4°C) TEMED 8 room temprature Antibodies Primary antibody: 5A11(anti-mouse IgG) 500x-1000x Secondary