Seracare特约代理商|0730-0003 Serase

在RT-PCR中避免DNA污染（Avoiding DNA Contamination in R

was isolated according to protocol by five different methods. 0.5 µg RNA was used in RT-PCR reactions with Ambion's RETROscript® Kit. PCR reactions were performed with 5 µg RNA. 10 µl of each reaction was electrophoresed on a 2% agarose gel and stained

Genomic DNA Extraction - PureLink

protein denaturation. Add 200 µl 96-100% ethanol to the lysate. Mix well by vortexing for 5 seconds. Proceed to Binding DNA Preparing Mammalian Tissue Lysate Procedure to prepare lysate from mammalian tissues is described

Differential Display of RNAs

x PCR reaction buffer, 1.6 µl 4dNTP mix (250 pmol/µl), 2 µl T12 MN primer, 2 µl arbitrary primer (decamer), 0.2 µl Taq DNA polymerase (5 U/µl) use the PCR conditions as under section III. run PCR product on a 1.5 % agarose gel