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文献和实验Isolation of RNA from Difficult Tissues
ToTALLY RNA™ Kit). Note that many of these techniques can be used with other RNA isolation protocols as well. Heart and Skeletal Muscle: Fibrous Tissues For fibrous tissues such as rat and mouse heart and skeletal muscle, the most difficult step
with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™
Quantitation of Multiple RNA Species
the elimination of differences in reaction mix volumes and conditions that may occur in separate samples, but importantly it also allows reference of RNA quantitation to an internal control (also see Notes 1 and 2 ). Internal controls are RNA species
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